A Manual of Chemical & Biological Methods for Seawater by Timothy Richard Parsons

By Timothy Richard Parsons

An advent to the quantitative research of seawater, describing intimately organic and chemical options, that are thought of to be among these typically utilized by organic oceanographers. The handbook offers whole directions for the addition of reagents and calculation of effects in regards fabric for every procedure in order that the unique texts may be consulted if worthy. generally, the options require no less than previous expert education and techniques desiring very dear apparatus were refrained from

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2. Aerate the sample in the tube with highly pure nitrogen gas for 5 min and seal the tube (Note a). 3. Let stand for 22 hr at 110°C in order to hydrolyze the dissolved combined amino acids in the seawater sample. 4. Cool the sealed ampule with tap water and open the ampule after hydrolysis. 5. Transfer the hydrolyzate into a 25-ml capacity rotary evaporator flask and evaporate to dryness at a temperature below 40°C. 6. 02 N N a O H into the flask in order to remove ammonia in the hydrolyzate and evaporate to dryness again at a temperature below 40°C (Notes b, c).

8. Add 1 ml of acetone and mix. 9. 8:1 of watenacetone mixture at a wavelength of 635 nm. 10. Correct the measured extinction by subtracting the analytical blank (see Section G). Calculate the total monosaccharide concentration in water samples in /imoles/1 from the expression, ^mole/1 = (Es — Eh)xF, where Es is the mean extinction of triplicate analyses of water samples, Eh is the mean extinction of duplicate analyses of analytical blanks and F i s determined as described below. G. 0 ml portions (in duplicate) of the sample are reduced with borohydride and the p H adjusted as described in Sections F - l and F - 2 .

1 ml of periodic acid solution into the sample solution and let stand for 10 min at room temperature in the dark. In this reaction, two hydroxyl groups of alditol are oxidized to aldehyde groups. 4. 1 ml of sodium arsenite solution into the sample solution in order to stop the oxidation reaction. Let stand for at least 10 min in the dark (Note e). 5. 2 ml of 2N HC1 into the sample and allow the amber color to disappear. 6. 2 ml of M B T H reagent into the sample solution. Allow the tightly-capped tubes to stand for 3 min in a boiling water bath.

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