Antibiotics by John H. Hash

By John H. Hash

The severely acclaimed laboratory normal, tools in Enzymology, is without doubt one of the so much hugely revered guides within the box of biochemistry. considering the fact that 1955, every one quantity has been eagerly awaited, usually consulted, and praised via researchers and reviewers alike. The sequence includes a lot fabric nonetheless appropriate this present day - actually a necessary book for researchers in all fields of lifestyles sciences.

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D. Semaka, H. M. Van den Elzen, J. E. Kinnear, and R. L. S. Whitehouse, Antimicrob. Ag. Chemother. 3, 625 (1973). [3] METHODS FOR STUDY OF ANTIBIOTIC R E S I S T A N C E PLASMIDS 45 ent strain (a nalidixie acid resistant strain, for example) are gently stabbed into the surface of a rich agar plate (containing no selective agents). The colonies should be well separated, about 50 or so can be put on a standard sized petri dish. Colonies of putative R + strains are then stabbed into the recipient colonies, in turn, using sterile toothpicks.

21 [35]. 1~T. Watanabe and T. Fukasawa, Y. Bacteriol. 82, 202 (1961). 1, Mitsuhashi,2 pp. 51-54. 18R. Nakaya, A. Nakarnura, and T. Murata, Biochem. Biophys. Res. Commun. 3, 654 (1960). 1~R. Nakaya and R. Rownd, ]. Bacteriol. 106, 773 (1971). 20R. P. Novick, Virology 33, 155 (1967); K. Smith and R. P. Novick, Y. Bacteriol. 112, 761 (1972). 21R. P. Novick, Bacteriol. Rev. 33, 210 (1969). ~2R. P. Novick and D. Bouanchaud, Ann. Y. Acad. Sci. 182, 279 (1971). 46 METHODS FOR THE STUDY OF ANTIBIOTICS [3] the limited capacity of the transducing bacteriophages for DNA; these deletions can then be used in the deletion-mapping of point mutations.

3, 625 (1973). [3] METHODS FOR STUDY OF ANTIBIOTIC R E S I S T A N C E PLASMIDS 45 ent strain (a nalidixie acid resistant strain, for example) are gently stabbed into the surface of a rich agar plate (containing no selective agents). The colonies should be well separated, about 50 or so can be put on a standard sized petri dish. Colonies of putative R + strains are then stabbed into the recipient colonies, in turn, using sterile toothpicks. The colonies m a y be numbered on the back of the plate with a marker.

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