By Jeffrey D. Hosenpud, Barry H. Greenberg
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Low-energy CID favors peptide fragmentation at (−CO-NH), a bond that links two amino-acid residues, which primarily generates ions of b and y types. Different instrument types and configurations allow for the detection of different type of fragment ions. Fragmentation can be performed either in the same device where the ions are trapped or in a separate collision cell. Depending on the type of the analyzer, tandem analysis 22 J. Cosme et al. can either be performed “in space”, by combining several analyzers, or “in time”, by using the same ion-trapping device to perform different stages of analysis sequentially.
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