Dendritic Cell Protocols by Elodie Segura, Nobuyuki Onai

By Elodie Segura, Nobuyuki Onai

The 3rd variation of this quantity is geared toward offering either newbies and more matured researchers a call of ways to isolate and study dendritic cells(DC). An introductory evaluate presents an outline of modern advances within the characterization of DC subsets in mouse and human. whereas extra chapters offer the way to tradition human and mouse dendritic cells, protocols for the isolation of dendritic cells, the isolation of dendritic telephone progenitors from mouse, and the purification of dendritic cells from human blood. Written within the hugely winning Methods in Molecular Biology series structure, chapters comprise introductions to their respective issues, lists of the required fabrics and reagents, step by step, comfortably reproducible laboratory protocols, and tips about troubleshooting and warding off recognized pitfalls.


Authoritative and cutting-edge,  Dendritic phone Protocols, 3rd version aims to make sure winning ends up in the extra research of this very important field.

Show description

Read Online or Download Dendritic Cell Protocols PDF

Best anatomy & physiology books

Biophysical Thermodynamics of Intracellular Processes: Molecular Machines of the Living Cell

The most target of this ebook is to explain in actual phrases the unusual gains of "machines" having molecular dimensions that play the valuable position within the most vital organic methods, viz. , power transduction and enzyme catalysis. for the reason that those molecular engines paintings with thermal, chemical, and mechanical strength, the perfect framework to debate them comes from thermodynamics and chemical kinetics.

The Equine Distal Limb: An Atlas of Clinical Anatomy and Comparative Imaging

Jean-Marie Denoix is the world’s major equine musculoskeletal procedure anatomist and has turn into one of many preferable equine diagnostic ultrasonographers. there's consequently not anyone higher to bring together a reference atlas of the medical anatomy of the foot, pastern and fetlock, correlated with photos bought by way of radiography, diagnostic ultrasonography and magnetic resonance imaging.

Platelets: Receptors, Adhesion, Secretion Part B

This quantity encompasses a finished choice of equipment for the isolation of platelet membranes, subcellular organelles, the cytoskeleton, and for the assay and purification of platelet receptors. Key gains* Platelets are mobile components with the top density of receptors consistent with membrane floor quarter focused on binding of adhesive molecules, clotting, enzymes, and vasoactive amines* Platelets are crucial for the arrest of bleeding and for formation of intravascular thrombi contributing to middle assaults, strokes, and disseminated intravascular coagulation, and are taken with immune advanced illness* Platelets are important for learning the techniques of mobilephone adhesion, neurotransmitter uptake, stimulus-response coupling, together with sign transduction and secretion

Advances in Morphometrics

This quantity relies at the NATO complex research Institute, "Advances in Mor­ phometries" held in eleven Ciocco, Tuscany, Italy from July 18-30, 1993, and directed by means of Leslie F. Marcus. The "Advances in Morphometries" ASI was once marketed in Nature and a couple of expert journals. bulletins have been despatched to suitable associations and departments during the international.

Extra resources for Dendritic Cell Protocols

Example text

2 Freezing MuTu DC Lines 1. Place the freezing vials at 4 °C 1 h before use. 2. Dissociate the cells by incubating them for 10 min in cold cell dissociation buffer. 3. Centrifuge the cells at 360 × g for 5 min. 4. Label the precooled freezing vials (see Note 16). 5. Gently dissociate the dry pellet. 6. Resuspend the cells at 3 × 106/mL in ice-cold freezing medium. 7. Transfer the cells to the vials (1 mL per vial). Close the tubes well. 8. Place the tubes quickly in the freezing container and place the container at −70 °C (see Note 17).

Ahrens S, Zelenay S, Sancho D, Hanc P, Kjaer S, Feest C, Fletcher G, Durkin C, Postigo A, Skehel M, Batista F, Thompson B, Way M, Reis e Sousa C, Schulz O (2012) F-actin is an evolutionarily conserved damage-associated molecular pattern recognized by DNGR-1, a receptor for dead cells. Immunity 36(4): 635–645 11. Sancho D, Joffre OP, Keller AM, Rogers NC, Martinez D, Hernanz-Falcon P, Rosewell I, Reis e Sousa C (2009) Identification of a dendritic cell receptor that couples sensing of necrosis to immunity.

3. FACS buffer: PBS, 1 mM EDTA, 10 mM HEPES. 4. Staining buffer (SB): FACS buffer, 2 % FCS. 5. Human TruStain FcX™ (Fc Receptor Blocking Solution, BioLegend). 6. , 50 μL TruStain FcX™ for 1 mL SB). 7. LIVE/DEAD® Fixable Aqua Dead Cell Stain Kit (Invitrogen). 8. 5 % paraformaldehyde working solution: prepare 4 % (w/v) stock solution in PBS, adjusted to pH 7, according to manufacturer’s instructions. Stock solution should be aliquoted in 10 mL volumes in 15 mL polypropylene tubes and frozen at −20 °C.

Download PDF sample

Rated 4.01 of 5 – based on 31 votes