Platelets: Receptors, Adhesion, Secretion Part B by Jacek J. Hawiger (Eds.)

By Jacek J. Hawiger (Eds.)

This quantity features a finished selection of tools for the isolation of platelet membranes, subcellular organelles, the cytoskeleton, and for the assay and purification of platelet receptors.

Key Features
* Platelets are mobile components with the top density of receptors consistent with membrane floor zone taken with binding of adhesive molecules, clotting, enzymes, and vasoactive amines
* Platelets are crucial for the arrest of bleeding and for formation of intravascular thrombi contributing to center assaults, strokes, and disseminated intravascular coagulation, and are serious about immune advanced disease
* Platelets are helpful for learning the tactics of mobile adhesion, neurotransmitter uptake, stimulus-response coupling, together with sign transduction and secretion

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Platelets: Receptors, Adhesion, Secretion Part B

This quantity encompasses a accomplished choice of tools for the isolation of platelet membranes, subcellular organelles, the cytoskeleton, and for the assay and purification of platelet receptors. Key gains* Platelets are mobile components with the top density of receptors in step with membrane floor quarter interested in binding of adhesive molecules, clotting, enzymes, and vasoactive amines* Platelets are crucial for the arrest of bleeding and for formation of intravascular thrombi contributing to middle assaults, strokes, and disseminated intravascular coagulation, and are concerned with immune advanced affliction* Platelets are precious for learning the strategies of mobile adhesion, neurotransmitter uptake, stimulus-response coupling, together with sign transduction and secretion

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Extra info for Platelets: Receptors, Adhesion, Secretion Part B

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3 M. J. Broekman, R. I. Handin, A. Derksen, and P. Cohen, Blood 47, 963 (1976). 4 K. L. Kaplan, M. J. Broekman, A. Chernoff, G. R. Lesznik, and M. Drillings, Blood 53, 604 (1979). 5 M. B. Zucker, M. W. Mosesson, M. J. Broekman, and K. L. Kaplan, Blood 54, 8 (1979). 6 M. B. Zucker, M. J. Broekman, and K. L. Kaplan, J. Lab. Clin. Med. 94, 675 (1979). / S. T. Silk, K. T. H. Wong, and A. J, Marcus, Biochemistry 20, 391 (1981). 8 M. J. D. Thesis, p. 65 (1976). METHODS IN ENZYMOLOGY, VOL. 215 Copyright © 1992 by Academic Press, Inc.

The yield of membranes by this technique is approximately 3 mg/unit of platelet concentrate, or about 50% increase over the original procedure. Purity is equal to that of the original procedure based on marker enzymes for plasma membranes. The procedure has also been used satisfactorily in our hands for the preparation of plasma membranes following proteolysis of intact platelets with Serratia marcescens protease. [5] I s o l a t i o n o f D e n s e G r a n u l e s f r o m H u m a n P l a t e l e t s By MmIAM H.

H. F u k a m i , Arch. Biochem. Biophys. 153, 726 (1972). The valve modification described facilities regulation of the flow rate but is not essential. 7 M. H. F u k a m i , J. S. Bauer, G. J. Stewart, and L. Salganicoff, J. CellBiol. 77, 389 (1978). 8 D. D. Tyler and J. Gonze, this series, Vol. 10, p. 75. 9 A. L. Smith, this series, Vol. 10, p. 84. 10 R. E. Basford, this series, Vol. 10, p. 98. it O m i s s i o n o f nagarse gives preparations that are difficult to r e s u s p e n d and do not separate well on s u c r o s e density gradient centrifugation.

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